F4424
Sigma
Monoclonal Anti-Fas (CD95/Apo-1) antibody produced in mouse
clone DX2, purified immunoglobulin, buffered aqueous solution
| Synonym: | Anti-Apo-1, Anti-CD95, Monoclonal Anti-Fas |
Description
| Immunogen | murine L cells transfected with a human Fas/CD95 cDNA. |
| Specificity | Reacts specifically with the functional epitope of human Fas (CD95/Apo-1) antigen. By immunoblotting, the clone recognizes denatured, non-reduced recombinant human Fas (amino acid residues 1-173). The antibody is reactive in flow cytometry, and may be reactive in the induction of apoptosis. |
| Physical form | Solution from a bioreactor culture supernatant in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | purified immunoglobulin |
| clone | DX2 (monoclonal) |
| form | buffered aqueous solution |
| usage | 10 μL sufficient for 1 × 106 cells fluorescence intensity and maximum percentage positive similar to that obtained with saturating antibody levels |
| species reactivity | human |
| application(s) | flow cytometry: 4-20 μg/mL using cultured human Burkitt’s lymphoma Raji cells |
| microarray: suitable | |
| isotype | IgG1 |
| Gene Information | human ... FAS(355) |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
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References
| reference | Sciacqua, A., et al., Interleukin-6 Impairs the Insulin Signaling Pathway, Promoting Production of Nitric Oxide in Human Umbilical Vein Endothelial Cells Mol. Cell. Biol. 27, 2372-2383, (2007) Abstract |
| Cifone, M.G. J. Exp. Med. 177, 1547, (1993) | |
| Lanier, L.L., et al. J. Immunol. 153, 2417, (1996) | |
| Choe, J., et al. J. Immunol. 157, 1006, (1996) | |
| Stohl, et al. J. Immunol. 160, 5231, (1998) | |
| Caricchio, R., J. Immunol. 161, 241, (1998) |
